| Magnificent Mulberries |
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| Written by The Clever Chromatographer | |||||
| Saturday, 12 December 2009 15:53 | |||||
This time I would like to highlight an article published in the in Journal of Food Composition and  Analysis entitled “The composition of anthocyanidins in mulberry and their antioxidant activity.” It took me a while to come across this article because it is found in a journal that does not normally carry CCC/CPC articles. I am attracted to the article because I have fond memories of picking and eating mulberries while growing up on the farm. Mulberry trees grew all over the place in southern Minnesota because they seeds were transported through bird feces. In this article, the authors report the separation of five anthocyanins with CCC. This is not terribly unusual, CCC has been very effective in studying anthocyanin plant pigments. What caught my eye was that 2 pair of epimers were separated: cyanidin 3-O-(6"-O- α-rhamnopyranosyl-β-glucopyranoside) and cyanidin 3-O-(6"-O- α-rhamnopyranosyl-β-galactopyranoside) as well as cyanidin 3-O-β-glucopyranoside and cyanidin 3-O-β-galactopyranoside. The separation of complex molecules that only differ by the stereochemical orientation of one hydroxyl group is pretty amazing. I noted with satisfaction that these two pairs of epimers were not separable with HPLC. I have seen too many articles where the compounds obtained by CCC/CPC are easily baseline separated with column chromatography methods.
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| Last Updated on Sunday, 16 May 2010 07:04 |