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Pulsed Liquid-Liquid Chromatography |
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Written by The Clever Chromatographer
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Sunday, 22 November 2009 18:14 |
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A recent article in JCA volume 1216 pp7761-7766 “Support-free pulsed liquid-liquid chromatography” pushes the boundary of the counter-current technique. Basically, the mobile phase is pumped through a series of coils, much like CCC. Instead of centrifugal motion retaining the stationary phase in the column, a pulsing device situated at the mobile phase entrance creates a continuous back-and-forth movement of the solvents in the column. As I understand it, the pulsing creates a localized counter-current movement in the coils. Therefore, the authors could have chosen “pulsed counter-current chromatography” as well as “pulsed liquid-liquid chromatography.” The coils were fashioned from glass tubing so that the phase mixing could be directly observed. Tests showed that the amplitude and frequency of the pulse play the role of rotation speed in CCC. The more and greater pulsing the more stationary phase is retained. The flow rate plays same role in Pulsed CCC as in CCC: the higher flow rate decreases Sf. Luckily, the authors do not propose an acronym (PLLC or PCCC?) for their new technique.
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Last Updated on Sunday, 22 November 2009 18:16 |
