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Reduce, Reuse, and Recycle |
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Written by The Clever Chromatographer
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Monday, 26 October 2009 18:39 |
A paper that I have had in my folder for a few years now is “Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography” JCA 1127 (2006) pp. 298-301. Recycling HPLC has been around for decades but, as far as I know, it is a relatively little-used technique in column chromatography. It is a very-much simplified version of the very common prqactice collecting, combining, concentrating, and rechromatographing fractions. My guess as to why recycling is not used more often in HPLC is 1) the sample volume increases significantly on each cycle and 2) recycling requires an isocratic elution solvent system. CCC/CPC has the distinct advantage in both of these areas: eluted samples tend to be concentrated and the normal mode of elution is isocratic. In this article the preparative separation of gambogic acid and epigamboic acid was achieved in a hexane-methanol-water solvent system after 6 cycles. The plumbing for recycling CCC is much easier than tandem (so-called “two-dimensional”) CCC and only one instrument is required. The separation process can be easily monitored by running the eluant through the detector flow cell each time. 
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Last Updated on Monday, 26 October 2009 18:47 |
