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Written by The Clever Chromatographer
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Saturday, 04 February 2012 19:56 |
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While you might have heard that “CEO” stands for Corporate Executive Officer, a recent article in Analytica Chimica Acta entitled “Preparative isolation and purification of cuminaldehyde and p-menta-1,4-dien-7-al from the essential oil of Cuminum cyminum L. by high-speed counter-current chromatography.“ gives another aspect of this abbreviation. The article describes ability of CCC to separate monoterpenes in a complex essential oil mixture. A nice puzzle presented itself. Cumin essential oil (CEO) has been studied numerous times by GC, four references of previous studies were given in the article, but there is a major compound which has not been clearly characterized. In the authors’ own words, “there was an additional compound representing as much as 30% of CEO but being identified in each study as a different chemical, and its structure was identified by GC-MS.” After testing eight different solvent systems, a hexane/methanol/water solvent system was chosen to pull apart cuminaldehyde and its congener p-menta-1,4-dien-7-al with shake-flask K values of 1.29 and 1.63 respectively. Properly purified, the monoterpenes could be unambiguously characterized by NMR. The moral of the story is that GC-MS is not sufficient for the structural characterization of even relatively simple compounds. Nothing quite replaces having the purified compound in a vial when it comes to structural identification. This is the first CCC paper that I have seen in Analytica Chimica Acta in a long time.
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Written by Administrator
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Monday, 06 April 2009 06:56 |
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Dear User: This site was launched to provide a networking opportunity for all scientists and practitioners of counter-current chromatography, as well as those interested in modern liquid-liquid separation technology. While the resources on this website are in the process of being built, this menu provides links to external sites that offer significant information about CCC technology. Eventually, INCCC.NET intends to serve as a comprehensive, global resource for this purpose. While these activities are ongoing, "INCCC resources" are supplemented with information from external links.
The INCCC webteam |
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Last Updated on Monday, 06 April 2009 15:40 |
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