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Written by The Clever Chromatographer
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Sunday, 28 February 2010 18:08 |
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A recent article in the Journal of Chromatography A 2009, vol 1216, pages 6162-6169 entitled “Purification of Drugs from Biological Fluids by Counter-Current Chromatography” describes the use to CCC to separate a variety of drug molecules from crude bile and human serum. The use of on-line ion selective MS allows the authors to detect minute quantities of these drugs as they are eluted from a 17 mL column at 1 mL/min. They used a sequence of 20 mL mobile phase (elution), 20 mL stationary phase (extrusion and refilling column with stationary), and 20 mL mobile phase (re-equilibration) between injections. Interestingly, normal phase elution was employed for 3 of the 4 solvent systems they tried (HEMWat 5:5:5:5, HEMWat 7:3:5;5, EMWat 3:1:2, and ChMWat 7:13:8). For a 4 mL mobile phase volume the K coverage would be 0 to 1.5 (4 to 24 minutes) before extrusion. The drugs they tested had retention times of 9.0, 19.7, 21.3, 23.3, 38.0, 39.8, 41.2, and 41.3 minutes which means that half of them eluted in the extrusion phase. They also did a shake-flask partition study to determine which solvent system they would use for separation. I, however, could not get their shake-flask K values to match up very well with the CCC retention times. All in all a nice application that highlights an important advantage of CCC: it can be used with crude biological fluid samples. Selected ion MS works very well to find those molecules that you know must be eluting somewhere before the end of extrusion. This article was also reviewed by the folks at Cherry Instruments http://cherryinstruments.com/blog/?p=102
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Last Updated on Sunday, 28 February 2010 18:22 |
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Written by Administrator
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Monday, 06 April 2009 06:56 |
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Dear User: This site was launched to provide a networking opportunity for all scientists and practitioners of counter-current chromatography, as well as those interested in modern liquid-liquid separation technology. While the resources on this website are in the process of being built, this menu provides links to external sites that offer significant information about CCC technology. Eventually, INCCC.NET intends to serve as a comprehensive, global resource for this purpose. While these activities are ongoing, "INCCC resources" are supplemented with information from external links.
The INCCC webteam |
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Last Updated on Monday, 06 April 2009 15:40 |
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