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Written by The Clever Chromatographer
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Saturday, 14 January 2012 18:17 |
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A recent article in the Analytical and Bioanalytical Chemistry entitled “High-speed counter-current chromatographic separation of phytosterols” brings out several interesting aspects of CCC. Overall, the authors purify beta-sitosterol and sitostanol from a commercial crude beta-sitosterol standard from Merck. In addition, 11 minor phytosterols were identified in the same mixture by MS. Amazingly 29 solvent systems composed of either hexane/ethyl acetate/acetontrile or hexane/methanol/water were evaluated for their stability at 20, 35, 40, and 45 degrees C. Some solvent systems no longer separated into two layers at higher temperatures. The K values of 4 different phytosterols were determined in each solvent system as well. A solvent system composed of hexane/methanol/1% aqueous silver nitrate 34/24/1 was employed for the separation. Silver nitrate is believed to interact with unsaturated hydrocarbons. The mass of each fraction collected from the HSCCC was recorded. A series of fractions containing both beta-sitosterol and sitostanol were rechromatographed in the same solvent system and gave a baseline separation. Fractions containing a mixture of phytosterols were silylated, separated and characterized by GC and electron ionization MS.
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Written by Administrator
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Monday, 06 April 2009 06:56 |
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Dear User: This site was launched to provide a networking opportunity for all scientists and practitioners of counter-current chromatography, as well as those interested in modern liquid-liquid separation technology. While the resources on this website are in the process of being built, this menu provides links to external sites that offer significant information about CCC technology. Eventually, INCCC.NET intends to serve as a comprehensive, global resource for this purpose. While these activities are ongoing, "INCCC resources" are supplemented with information from external links.
The INCCC webteam |
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Last Updated on Monday, 06 April 2009 15:40 |
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